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Email the author (Login required) Growth, survival, and modulation of the intestinal microbiota of shrimp Penaeus vannamei fed with probiotic actinomycetes and highly diluted bioactive compounds
Submited: 2024-06-05 10:47:46 | Published: 2025-04-30 20:11:45
DOI: https://doi.org/10.3856/vol53-issue2-fulltext-3292
Abstract
The effect of actinomycetes probiotics and highly diluted bioactive compounds (HDBC) over the productive parameters, Vibrio and heterotroph counts, and gut microbiota composition of Penaeus vannamei juveniles was assessed. Four treatment and two control groups of 150 shrimps each received food containing T1 (Streptomyces sp. RL8 + Streptomyces sp. N7); T2 (HDBC); T3 (HDBC + Streptomyces sp. N7); T4 (HDBC + Streptomyces sp. RL8); C1 (distilled water) and C2 (water-diluted ethanol) for 30 days. The microbiota analysis was performed by sequencing the V3 region of the 16S rRNA gene using the Illumina platform. A significant increase in shrimp size was observed for T1 (3.74 ± 0.9 cm) and T2 (3.92 ± 0.93 cm), as well as in weight gain for T2 (3.15 ± 0.74 g). The greatest survival was achieved by T1 and T3, with 99.33% each. T1 and T2 also exhibited a significantly lower Vibrio count of 2.36 ± 0.11 and 2.43 ± 0.11 as well as of 1.85 ± 0.12 and 1.81 ± 0.29 in the rearing water and shrimp hepatopancreas, respectively. All treated groups showed high heterotroph counts in these two niches. The gut microbiota of shrimps was diverse and comprised mainly of Proteobacteria, Bacteroidetes, Verrucomicrobia, and Actinobacteria, with an average relative abundance of 54.46, 26.26, 11.14, and 5.54%, respectively. The specific genus Prevotella was stimulated by T1, Acinetobacter by T2, and Bacteroides and Enhydrobacter by T3. Streptomyces probiotics (T1) and HDBC (T2), but not the combination of Streptomyces spp. with HDBC (T3, T4), exhibited the best overall effects in juveniles of Penaeus vannamei.
