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Email the author (Login required) Stabilization of lyophilized bacteriophage vB_Pd_PDCC-1 with biopolymers for long-term storage under thermal stress
Submited: 2025-09-27 02:51:42 | Published: 2026-04-14 11:10:34
DOI: https://doi.org/10.3856/vol54-issue2-fulltext-3540
Abstract
The present work evaluated the combination of biopolymers used as preservative agents to enhance the viability and efficacy of the vB_Pd_PDCC-1 phage stored as a powder under high-temperature conditions. In the tests, phages were freeze-dried in a polymeric matrix with varying molar ratios of sodium alginate, gelatin, and trehalose, stored at 4, 25, 35, and 45°C, and evaluated at 1, 30, 60, and 90 days post-freeze-drying. The results indicated that the best treatment was the combination of trehalose (4% w/v) and gelatin (1.5% w/v), as it maintained phage viability (log 6) for up to 90 days at 45°C. Likewise, phage therapy trials using Artemia nauplii inoculated with Vibrio diabolicus and lyophilized phages embedded in biopolymeric matrices showed survival rates similar to those of the control group, reducing bacterial concentration by 3 log orders of magnitude. The study identified the combination of trehalose and gelatin as a promising additive mixture for protecting the powdered bacteriophage Pd_PdCC-1.
